SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Effects of Formaldehyde and Vimentin Expression in Rat Skin

Nascimento,R.S.; Santiago,M.F.; Marques,S.A.; Allodi,S.; Martinez,A.M.B..

Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Satellite glial cells; Peripheral nervous system; Vimentin; S-100; Electron microscopy.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100011

Expression of Vimentin and GFAP Protein of Cerebral Cortex and Its Impact on Corticogenesis Disorder as a Result of 2-Methoxyethanol

Kalkanli,Sevgi; Kalkanli,Nevin; Deveci,Engin; Dal,Tuba; Baran,Özlem.

The Wistar rats ( 9 weeks old, 180200 g body weight) used in these trials were divided into two groups of 16 animals each (Control group and Experimental group). 100x65x100 in the sizes of the experimental group were taken into a glass vase. During the time period of 8 weeks, 5 days a week with 8 hours the inhalation of 10 ppm formaldehyde was made. Skin was removed and placed in 10% formaline. Sections were stained with Hematoxylene-Eosine, Trichrome-Masson and observed under light microscope. In this study, histopathological and immunohistochemical techniques due to the impact of the changes in formaldehyde and examined the distribution of vimentin.

Tipo: Journal article

Palavras-chave: Formaldehyde; Skin; Vimentin.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300011

Irnidayanti,Yulia; Darmanto,Win.

One of the plastic base material, widely used in the plastics industry in various countries, is a ester phthalate. These compounds will be oxidizedin the body to 2-methoxyethanol (2-ME). Effect of 2-ME on human health and the environment depends on the number, duration and frequency of exposure. 2-ME and its metabolites in the body can damage cells and tissues. The body can be exposed by 2-ME through the air, water and soil. Western blot results showed that the protein Vimentin was detectable in the control group at GD-11 to 17, meanwhile GFAP protein was detachable in the control group atGD- 12 to GD-18. After administration 2-ME, the expression of Vimentinprotein were changed, and started at GD- 12 up to GD-18. whereas the expression of GFAP protein...

Tipo: Journal article

Palavras-chave: 2-methoxyethanol; Vimentin; GFAP; Cerebral cortex; Brain.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300003

Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation

Gomes,F.C.A.; Paulin,D.; Moura Neto,V..

Intermediate filament (IF) proteins constitute an extremely large multigene family of developmentally and tissue-regulated cytoskeleton proteins abundant in most vertebrate cell types. Astrocyte precursors of the CNS usually express vimentin as the major IF. Astrocyte maturation is followed by a switch between vimentin and glial fibrillary acidic protein (GFAP) expression, with the latter being recognized as an astrocyte maturation marker. Levels of GFAP are regulated under developmental and pathological conditions. Upregulation of GFAP expression is one of the main characteristics of the astrocytic reaction commonly observed after CNS lesion. In this way, studies on GFAP regulation have been shown to be useful to understand not only brain physiology but...

Histone demethylase retinoblastoma binding protein 2 regulates the expression of α-smooth muscle actin and vimentin in cirrhotic livers

Palavras-chave: CNS; Astrocyte; Growth factors; Intermediate filaments; GFAP; Vimentin; GFAP gene.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500016

Wang,Q.; Wang,L.X.; Zeng,J.P.; Liu,X.J.; Liang,X.M.; Zhou,Y.B..

Liver cirrhosis is one of the most common diseases of Chinese patients. Herein, we report the high expression of a newly identified histone 3 lysine 4 demethylase, retinoblastoma binding protein 2 (RBP2), and its role in liver cirrhosis in humans. The siRNA knockdown of RBP2 expression in hepatic stellate cells (HSCs) reduced levels of α-smooth muscle actin (α-SMA) and vimentin and decreased the proliferation of HSCs; and overexpression of RBP2 increased α-SMA and vimentin levels. Treatment with transforming growth factor β (TGF-β) upregulated the expression of RBP2, α-SMA, and vimentin, and the siRNA knockdown of RBP2 expression attenuated TGF-β-mediated upregulation of α-SMA and vimentin expression and HSC proliferation. Furthermore, RBP2 was highly...

Immunohistochemical and Histopathological Changes in the Skin of Rats after Administration of Lead Acetate

Palavras-chave: RBP2; Liver cirrhosis; Fibrosis; Α-SMA; Vimentin; HSC.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900739

Leiomyosarcoma in a cockatiel

Sula,Bilal; Deveci,Engin; Özevren,Hüseyin; Ekinci,Cenap; Elbey,Bilal.

Lead acetate is a chemical compound. Sources of human exposure to this metal include many foods, drinking water and dust. The aim of this study was to determine the immunohistochemical and histopathological changes on the face skin after lead acetate application. Wistar Albino rats (180-200 g body weight) were divided into a controlled and lead acetate-exposed group. Rats received lead acetate at 500 ppm in their drinking water for 60 days. Both groups were fed with the same standard food, but lead acetate was added to the drinking water. During the experimental period, blood samples were drawn from the abdominal aorta of the anesthetised animals. At the end of exposure, body weight and blood lead levels were measured. Sections of rat facial skin were...

Tipo: Journal article

Palavras-chave: Lead acetate; Skin; Proliferating cell nuclear antigen; Vimentin.

Ano: 2016

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300017

Ciência Rural

Bonel,Josiane; Alberti,Taina dos Santos; Stumm,Geovana Kramer Fiala; Gamba,Conrado de Oliveira; Bandarra,Paulo; Cassali,Geovanni Dantas.

ABSTRACT: Cockatiels (Nymphicus hollandicus) are exotic birds thatoriginated from Australia.Because of their beauty and learning ability, they are one of the most popular pet birds among the Psittaciformes. The objective of this study was to report a case of leiomyosarcoma on the humeral musculature of the left wing of a cockatiel (Nymphicus hollandicus). The animal was admitted to the Wildlife Rehabilitation Center (NURFS-CETAS) of the Universidade Federal de Pelotas withswelling in the humeral region of the left wing. During surgery, the animal died and was transferred to the Laboratório Regional de Diagnóstico, Faculdade de Veterinária (LRD-UFPel). During histopathological evaluation (hematoxylin and eosin routine technique) of the tumor, spindle...

The Effects of Nicotine on the Incisive Teeth and Expression of Vimentin in Rats

Palavras-chave: Psittaciformes; Sarcomas; Alpha actin; Birds; Vimentin.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300550

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats

Faruk,E. R; Deveci,E; Kalkanli,S; Deveci,B.

Nicotine is an alkaloid toxic effects of oral cavity. In this study,14 adult Sprague-Dawley rats weighing 230-260 mg (±10 mg) were used as experimental animal. The rats of experimental group (n=6) were nicotinized systemically with nicotine sulphate, 2mg/kg subcutaneously, daily in period of 28 days. Pulp, alveolar bone tissue, periodontal membrane and gingival epithelial junction were investigated in these regions in incisive teeth longitudinal cross-section. Thinning of the collagen fibers in the pulp tissue, vascular congestion, and inflammatory cell infiltration were observed. Mesenchymal tissues that is stained positive for vimentin lay underneath the epithelium. A strong expression of vimentin can be observed in formed periodontal ligament.

Tipo: Journal article

Palavras-chave: Nicotine; Rat; Vimentin.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200026

Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C..

The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...

Thyroid hormone regulates protein expression in C6 glioma cells

Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603